High Pressure Liquid Chromatography (HPLC)

High Pressure Liquid Chromatography (HPLC) is an important separation technique for compounds of low or no volatility thus it makes a nice complimentary procedure to GC.  Like GC and all other chromatographic techniques, in HPLC the separation is based on a partitioning of the sample components on a stationary phase and a mobile phase.  The liquid mobile phase is the variable.  Both the polarity and pH of the mobile phase can be varied.  We possess a Waters 510 HPLC equipped with a Waters 991 UV-VIS photodiode array detector.  This equipment was obtained with the aid of a 1995 National Science Foundation Grant written by Dr. Franz.

Aaron McGaw eagerly anticipates his HPLC results.

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Proceed to the description of the atomic absorption spectrometer.   

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Last updated September 13, 2007.
The URL for this page is http://www.lycoming.edu/chemistrydept/tour/inst8.htm