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Ultraviolet-Visible (UV-VIS) Spectrophotometry


Ultraviolet-Visible Spectrophotometry (UV-VIS) is useful for both quantitative and qualitative work.  The technique is based on the interaction of a sample compound with the ultraviolet and neighboring visible portions of the electromagnetic spectrum.  The absorption of light is associated with electronic transitions within the sample compound.  Our particular instrument is a Cary Bio 50 ultraviolet-visible spectrophotometer, acquired in 2002 with funding from the National Science Foundation.* The HPLC is also equipped with a diode-array UV-VIS detector.
 

Olivia English (l) and Michelle Morone (r) determine the UV-vis spectrum of a sample


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Last updated September 13, 2007. * This material is based upon work supported by the National Science Foundation under Grant No. 0116502
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