Ultraviolet-Visible Spectrophotometry (UV-VIS) is useful for both
quantitative
and qualitative work. The technique is based on the interaction of
a sample compound with the ultraviolet and neighboring visible portions
of the electromagnetic spectrum. The absorption of light is associated
with electronic transitions within the sample compound. Our particular
instrument is a Cary Bio 50 ultraviolet-visible spectrophotometer, acquired in
2002 with funding from the National Science Foundation. The HPLC
is also equipped with a diode-array UV-VIS detector.
Olivia English (l) and Michelle Morone (r) determine the UV-vis
spectrum of a sample
Return to the main instrument page
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Last updated October 14, 2004.
The URL for this page is http://www.lycoming.edu/chem/tour/inst5.htm
